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JoVE Journal Neuroscience
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
 

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Article DOI: 10.3791/64196-v • 06:55 min • August 4th, 2022
August 4th, 2022 •
*1,2, *1,2, 2,3,4
1TUM Medical Graduate Center, Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology, Technical University of Munich, 4Munich Cluster for Systems Neurology
* These authors contributed equally

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Resumo
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The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.

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Keywords: Microfluidics Axonal Mitochondria Neurodegenerative Diseases Fluidic Pressure Gradient Membrane Potential Hippocampal Neurons Cell Culture B-27 Neurobasal Media Microgrooves
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