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Bröst Transplantation av stromaceller och celler carcinoma in C57BL/6J Möss
JoVE Journal
Medicine
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JoVE Journal Medicine
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice
DOI:

13:01 min

August 12, 2011

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Chapters

  • 00:05Title
  • 01:33Isolation and Extraction of Donor Collagen from C57BL/6J Mice
  • 04:09Isolation and Culture of Donor Mammary Carcinoma Cells and Fibroblasts from Normal and PyVmT C57BL/6J Mice
  • 06:33Preparation of Collagen Embedded Cells for Grafting
  • 08:32Orthotopic Transplantation of Collagen Embedded Cells in C57BL/6J Mice
  • 10:40Representative Results

Summary

Automatic Translation

I den här rapporten visar vi ett system för att isolera och odla celler givare från mus juvret och orthotopically transplantera dessa celler i mottagarländerna möss att analysera stromaceller: epiteliala interaktioner under juvertumör utveckling.

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