Developmental Biology
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Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
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Summary
Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.
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Protein-protein Interactions Co-localization Intercellular Junction Proteins Co-immunoprecipitation Mammary Glands Development Breast Cancer Technique In Vivo Microscopic Slides Formaldehyde Fixation Phosphate-buffered Saline Hydrophobic Barrier Histology Chamber Bovine Serum Albumin-Tris-Buffered SalineRelated Videos
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