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Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
 

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Article DOI: 10.3791/3340-v • 14:50 min • October 27th, 2011
October 27th, 2011 •
1, 1, 1, 1, 1,2,3, 1, 1
1Regenerative Biology, Morgridge Institute for Research, 2Department of Cell & Regenerative Biology, University of Wisconsin, 3Department of Molecular, Cellular, & Regenerative Biology, University of California

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Here we describe a method for preparation of both single read and paired end Illumina mRNA-Seq sequencing libraries for gene expression analysis based on T7 linear RNA amplification. This protocol requires only 10 nanograms of starting total RNA and generates highly consistent libraries representing whole transcripts.

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MRNA-Seq Illumina Libraries Global Gene Expression Mutation Analysis Allele-specific Expression Genome-wide Analyses Non-sequenced Genomes Copy Numbers Illumina's Genome Analyzer Paired End Sequencing Alternate Splice Junctions Insertions And Deletions De Novo Transcriptome Assembly Limited Starting Material Nanograms Of Total RNA PCR Amplification Minimal PCR Amplification
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