Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Click here for the English version

Developmental Biology

Published: September 15, 2023 doi: 10.3791/65580

Materials

Name Company Catalog Number Comments
6-well culture dish  Falcon REF 353502 Air-Liquid Interface (ALI) Cultures  
Cell culture inset  Falcon REF 353090. 0.4 µm Transparent PET Membrane
Collagenase Type 1 Worthington Biochemical LS004196
Dulbecco’s modified Eagle’s medium  Corning 10-013-CV 4.5 g/L glucose
Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA) Sigma-Aldrich E5134
Fetal bovine serum ThermoFisher 16140-071
Glucose Sigma-Aldrich G8270
Hanks’s buffered saline solution Gibco 14170-112 No calcium, no magnesium
Penicillin/streptomycin  Gibco 15-140-122
Pogassium phosphate monobasic (KH2PO4) Sigma-Aldrich P5379
Potassium chloride (KCl) Sigma-Aldrich P9333
Sodium bicarbonate (NaHCO3) Sigma-Aldrich S6014
Sodium chloride (Nacl) EMD  CAS 7647-14-5
Sodium phosphate monobasic (NaH2PO4) Sigma-Aldrich S0751
Trypsin Gibco 27250-042

DOWNLOAD MATERIALS LIST

References

  1. Lucas, A. M., Stettenheim, P. R. Avian anatomy: Integument part I and part II. Agriculture Handbook. 362, Agricultural Research Service, U.S. Department of Agriculture, Washington D.C.. (1972).
  2. Sengel, P. In Morphogenesis of Skin, l-277. , Cambridge Univ. Press. New York. (1976).
  3. Dhouailly, D., Wilehm Roux, Formation of cutaneous appendages in dermo-epidermal recombinations between reptiles, birds and mammals. Archives of Developmental Biology. 177 (4), 323-340 (1975).
  4. Jiang, T. -X., Chuong, C. -M. Mechanism of feather morphogenesis: I. Analyses with antibodies to Adhesion Molecules Tenascin, N-CAM and Integrin. Developmental Biology. 150 (1), 82-98 (1992).
  5. Li, A., et al. Shaping organs by a Wnt / Notch / non-muscle myosin module which orients feather bud elongation. Proceedings of the National Academy of Sciences, USA. 110 (16), E1452-E1461 (2013).
  6. Li, A., et al. Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks. Nature Communications. 9 (1), 5377 (2018).
  7. Ting-Berreth, S. A., Chuong, C. M. Local delivery of TGF beta2 can restore epithelium dependent organization of mesenchymal condensation during skin appendage morphogenesis. Developmental Biology. 179 (2), 347-359 (1996).
  8. Widelitz, R. B., Jiang, T. -X., Noveen, A., Chen, C. -W. J., Chuong, C. -M. FGF induces new feather buds from developing avian skin. Journal of Investigation Dermatology. 107 (6), 797-803 (1996).
  9. Rawles, M. E. Tissue interactions in scale and feather development as studies in dermal-epidermal recombinations. Journal of Embryology and Experimental Morphology. 11, 765-789 (1963).
  10. McAleese, S. R., Sawyer, R. H. Correcting the phenotype of the epidermis from chick embryos homozygous for the gene scaleless (sc/sc). Science. 214 (4524), 1033-1034 (1981).
  11. Chuong, C. M., Widelitz, R. B., Ting-Berreth, S., Jiang, T. X. Early events during avian skin appendage regeneration: dependence on epithelial-mesenchymal interaction and order of molecular reappearance. J Invest Dermatol. 107 (4), 639-646 (1996).
  12. Jiang, T. X., et al. Global feather orientations changed by electric current. iScience. 24 (6), 102671 (2021).
  13. Jiang, T. X., Jung, H. S., Widelitz, R. B., Chuong, C. M. Self-organization of periodic patterns by dissociated feather mesenchymal cells and the regulation of size, number and spacing of primordia. Development. 126 (22), 4997-5009 (1999).
  14. Hamburger, V., Hamilton, H. A series of normal stages in the development of the chick embryo. Journal of Morphology. 188, 49-92 (1951).
  15. Chen, Y. P., et al. Conservation of early odontogenic signaling pathway in Aves. Proceedings of the National Academy of Sciences, USA. 97 (18), 10044-10049 (2000).
  16. Chuong, C. -M., Ting, S. A., Widelitz, R. B., Lee, Y. S. Mechanism of Skin Morphogenesis: II. Retinoic acid gradient modulates axis orientation and phenotypes of skin appendages. Development. 115 (3), 839-852 (1992).
  17. Noveen, A., Jiang, T. -X., Chuong, C. -M. Protein kinase A and protein kinase C modulators have reciprocal effects on mesenchymal condensation during skin appendage morphogenesis. Developmental Biology. 171 (2), 677-693 (1995).
  18. Wu, X. S., et al. Self-assembly of biological networks via adaptive patterning revealed by avian intradermal muscle network formation. Proceedings of the National Academy of Sciences, USA. 116 (22), 10858-10867 (2019).
Play Video
PDF DOI DOWNLOAD MATERIALS LIST

Cite this Article

Jiang, T., Secor, M., Lansford, R.,More

Jiang, T., Secor, M., Lansford, R., Widelitz, R. B., Chuong, C. M. Using Avian Skin Explants to Study Tissue Patterning and Organogenesis. J. Vis. Exp. (199), e65580, doi:10.3791/65580 (2023).

Less
Copy Citation Download Citation Reprints and Permissions
View Video

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter